The gut microbiota plays a significant role in the biotransformation of many endogenous molecules and xenobiotics. This process has a potential influence on the host's health and disease. Recent data clearly show that the gut microbiota is involved in the biotransformation of various substances, leading to a potential impact on the host's health. The optimization of the gut microbiome biotransformation is crucial for understanding the complex interactions between the host and the microbiota. This research activity aims to investigate the mechanisms underlying the gut microbiome biotransformation and its impact on the host's health.