Quantitative real-time polymerase chain reaction is an alternative method for the detection of HER-2 amplification in formalin-fixed paraffin-embedded breast cancer samples.
Copyright policy )Quantitative real-time polymerase chain reaction is an alternative method for the detection of HER-2 amplification in formalin-fixed paraffin-embedded breast cancer samples.
Fluorescent in situ hybridization (FISH) and immunohistochemistry (IHC) are the most common methods that are used to quantify HER-2 gene and protein levels, respectively, in human breast cancer. However, due to bad sample quality, some samples are unable to be subjected to a FISH assay. We evaluated 71 formalin-fixed paraffin-embedded (FFPE) breast carcinoma specimens by quantitative real-time polymerase chain reaction (qPCR), IHC, and FISH. We also performed qPCR and FISH assays on delayed formalin-fixed (DDF) samples. The qPCR results were in complete concordance with the results of IHC and FISH. In regards to the DDF samples, the HER-2 fluorescent signal seemed decayed compared with that of the DDF samples after 1 h. However, the qPCR method still works well up to 12 hours. Our results indicated that qPCR was obviously superior to FISH in cases that were not fixed in a reasonable amount of time. However, qPCR can be an alternative method by which to perform HER2 amplification assays in breast cancer.
- West China Hospital of Sichuan University China (People's Republic of)
Paraffin Embedding, Time Factors, Tissue Fixation, Receptor, ErbB-2, Gene Amplification, Reproducibility of Results, Breast Neoplasms, Real-Time Polymerase Chain Reaction, Immunohistochemistry, Gene Expression Regulation, Enzymologic, Workflow, Gene Expression Regulation, Neoplastic, Fixatives, Predictive Value of Tests, Formaldehyde, Biomarkers, Tumor, MCF-7 Cells, Humans, Female, In Situ Hybridization, Fluorescence
Paraffin Embedding, Time Factors, Tissue Fixation, Receptor, ErbB-2, Gene Amplification, Reproducibility of Results, Breast Neoplasms, Real-Time Polymerase Chain Reaction, Immunohistochemistry, Gene Expression Regulation, Enzymologic, Workflow, Gene Expression Regulation, Neoplastic, Fixatives, Predictive Value of Tests, Formaldehyde, Biomarkers, Tumor, MCF-7 Cells, Humans, Female, In Situ Hybridization, Fluorescence
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