Silencing Fas-associated phosphatase 1 expression enhances efficiency of chemotherapy for colon carcinoma with oxaliplatin.
Copyright policy )Silencing Fas-associated phosphatase 1 expression enhances efficiency of chemotherapy for colon carcinoma with oxaliplatin.
To investigate whether silencing Fas-associated phosphatase 1 (FAP-1) expression enhances the efficiency of chemotherapy for colon carcinoma with oxaliplatin.Expression of FAP-1 in mRNA and protein was detected by reverse transcription polymerase chain reaction (RT-PCR) and flow cytometry. Small interfering RNA (siRNA) was designed according to the FAP-1 mRNA sequence. Cell proliferation was evaluated by methyl thiazolyl tetrazolium (MTT) assay. Anenxin V- and propidine iodine (PI) were assayed by flow cytometry for the detection of apoptosis.The expression of FAP-1 was increased in SW480 cells after chemotherapy with oxaliplatin. Transfection of FAP-1 siRNA into SW480 cells silenced the expression of FAP-1 and consequently abolished the inhibitory function of Fas/FasL-mediated apoptosis pathway, thus increasing the efficacy of chemotherapy for colon carcinoma with oxaliplatin.RNA interference combined with conventional chemotherapy is more effective against colon cancer.
- Sun Yat-sen University China (People's Republic of)
- Sun Yat-sen Memorial Hospital China (People's Republic of)
- Hospital of Stomatology, Sun Yat-sen University China (People's Republic of)
Time Factors, Organoplatinum Compounds, Reverse Transcriptase Polymerase Chain Reaction, Protein Tyrosine Phosphatase, Non-Receptor Type 13, Antineoplastic Agents, Apoptosis, Genetic Therapy, Adenocarcinoma, Flow Cytometry, Transfection, Oxaliplatin, Chemotherapy, Adjuvant, Cell Line, Tumor, Colonic Neoplasms, Humans, RNA Interference, RNA, Messenger, Cell Proliferation
Time Factors, Organoplatinum Compounds, Reverse Transcriptase Polymerase Chain Reaction, Protein Tyrosine Phosphatase, Non-Receptor Type 13, Antineoplastic Agents, Apoptosis, Genetic Therapy, Adenocarcinoma, Flow Cytometry, Transfection, Oxaliplatin, Chemotherapy, Adjuvant, Cell Line, Tumor, Colonic Neoplasms, Humans, RNA Interference, RNA, Messenger, Cell Proliferation
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