????????: ???????????????? ?????????????????????????? ???????????? ?????????????????????? ????????????????????????, ????????????????????, ?????????????????????????? ?? ?????????????????????? ???????????????????????? ?????????????????? ?????????????????????????????????? ?????????????? ?????????? (T????)-??1 ???????????????? ???????? ???????????????? ???????????? ???????????????? (?????????? MCF-7 ?? T47D), ?? ?????????? ?????????????????????? ???????????????????????????????? ???????? ???????????? ?? T????-??1 ?? ?????????????????? ?????????????????????????????????? ????????????????????. ?????????????????? ?? ????????????: ?????? ?????????????????????? ???????????????????? T????-??1 ?? ???????????????????????????????????? ?????????? ???????????????????????? ???????????????????????????????? ????????????. ?????????????? ???????????? p53 ?? Bcl-2 ?????????????????? ?????????????? ??????????????????????????????. ????????????????????: ???????????? t47d, ?????????????? ?????????????????????????????????? ?????????????? ?????????????????????????????? ?? ?????????????????????????????????? ???????????????? T????-??1 ???? ?????????????????? ?? ???????????????? MCF-7, ?????????????????? ?????????? ?????????????????????????????? ?? ????????????????????. ???? ???????????????? ???????????????? ?????????? ?????????????????????????????????? ???????????? MCF-7 ?? T47D ?? ???????????????? ???????????????????????? ?????? ??????????????????????????, ???????? ???????????? T47D ???????? ?????????? ?????????????????????????????? ?? ??????????????????????. ?????????????????????? ?? ???????????????????? ???? ???????????? ???? ?????????????????? T????-??1 ???????????????? MCF-7 ?? T47D, ?????????? ?????? ???????????????????????? ?? ???????? 1???100 ??????/???? ?????????????????????? ???????????????? ?????????????????? T????-??1 . ???????????????????? ?????????? p53 ???????? ?????????? ?????????????????????? ?? ?????????????? T47D, ???? ?????????????????? ?? ???????????????? ?????????? MCF-7, ?? ???????? ?? ???????????? ???????????????? ?????????????????????????? ???? ???????????? ?????????? MCF-7 ???????? ???????????????? ???????????????????????? ?????????????????? ?????? ????????????. ?????? ???????? ?????????? Bcl-2 ?????? ?????????????? ???????????? ?? ?????????????? MCF-7. ????????????: ?? ?????????????????? ???????????? ?????????????????? ???????????????? ???????????? ???????????????? (MCF-7 ?? T47D) ????????????????????????, ?? ?????????????? ???? ?????????????????????? ?? ????????????????????????, ???????????????????????? ???????????????????? ?????????????????? ??????-??1 . ???????????????? ???????????????????????????? ??????-??1 ?? ?????????????? ???????????????? ?????????????????????????? ?? ?????????????????? ???????????????????? ??53 ?? ?????????????? MCF-7 ?????? ???????????????????? ?????????????????? ?????????????? ?????????? ?????????? ?? ?????????????? T47D. ???????????? ?????????? MCF-7, ?????????? ???????????????????????????? ?? ?????????????????????????????????? ???????????????? ?????????????????????? ??????-??1 , ?????? ???????????? ?????????? T47D, ???????????????????? ?? ?????????? ???????????????????? ?????????????????????????????????? ?? ???????????????? ????????????????????, ???? ???? ???????????????????? ???? ???????????????????????????????? ???? ???????????? ????????????????????????.

Aim: To compare the capability of methotrexate, cisplatin, doxorubicine and vincristine to induce production of the transforming growth factor b1 (TGF- b1) in two cell lines ??? MCF-7 and T47D ??? of human breast carcinoma, as well as to study sensitivity of these cells to TGF- b1 and mentioned anticancer drugs. Materials and Methods: ELISA for detection of TGF- b content in conditioned culture media and Western-blot analysis of the proapoptotic p53 and antiapoptotic Bcl-2 proteins were applied. Results: t47d cells showing higher resistance to growth inhibiting effect of TGF- b1 were also refractory to cisplatin. There was no difference between MCF-7 and T47D cells in their sensitivity to methotrexate and doxorubicine, although T47D cells were more sensitive to vincristine. It was found that methotrexate and vincristine did not affect TGF- b1 production, while doxorubicine used at a dose of 1???100 ug/ml, significantly induced TGF- b1 production in both cell lines. p53 expression in T47D cells was higher than in MCF-7 cells where only doxorubicin induced strongly p53 expression. It should be noted, that Bcl-2 was better expressed in MCF-7 cells, while it was almost undetectable in T47D cells. Conclusion: In cells of human mammary carcinoma of MCF-7 and T47D lines doxorubicine, unlike vincristine and methotrexate, in dose depending manner induces production of TGF- b1. TGF- b1 production in carcinoma cells was associated with doxorubicine-mediated p53 expression in MCF-7 cells or high basal level of p53 in T47D cells. The cells of MCF-7 line were more sensitive to growth inhibition by exogenous TGF- b1 and to cisplatine action than T47D cells, but there was no difference between these cell lines in sensitivity to other anticancer drugs.