The discovery of new genes with important regulatory functions on cellular level, allowed the investigation of many biological and molecular changes and defining their value as potential prognostic factors.The study included 122 patients with non-small cell lung cancer. We examined the expression of cyclin D1 and p53.The 5-years survival rate was 26% for the group with strong nuclear expression of cyclin D1, and 80% for the group with moderate expression of the marker. We found 5-years survival rate of 100% for the group with poor expression of p53, and 24.55% for the group with strong expression.The increased levels of cyclin D1 and p53 are negative prognostic factors for the patients with non-small cell lung cancer.