[PGM1 genotyping by PCR-SSCP].
[PGM1 genotyping by PCR-SSCP].
PGM1 genotyping by PCR-SSCP analysis.Amplified genome DNA from 156 unrelated Han individuals living in Wuhan, PCR products for exon 4 and exon 8 of PGM1 were then analyzed by SSCP to detect the genotypes.2 alleles and 3 genotypes were detected in exon 4 and 8 respectively. The discrimination power was 0.7318. PCR-SSCP analysis was suitable for determination of PGM1 genotypes from old blood and semen stains.PGM1 system typed by PCR-SSCP is useful for forensic identification.
- Wuhan Union Hospital China (People's Republic of)
China, Polymorphism, Genetic, Genotype, DNA, Polymerase Chain Reaction, Sensitivity and Specificity, Asian People, Gene Frequency, Phosphoglucomutase, Humans, Alleles, Polymorphism, Single-Stranded Conformational
China, Polymorphism, Genetic, Genotype, DNA, Polymerase Chain Reaction, Sensitivity and Specificity, Asian People, Gene Frequency, Phosphoglucomutase, Humans, Alleles, Polymorphism, Single-Stranded Conformational
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