FAK pathway regulates PGE₂ production in compressed periodontal ligament cells.
Copyright policy )FAK pathway regulates PGE₂ production in compressed periodontal ligament cells.
This study examined the role of focal adhesion kinase (FAK) as a mechanoreceptor in human periodontal ligament (hPDL) cells. hPDL cells were obtained from premolars extracted for orthodontic purposes. Mechanical stress was applied in a compressive manner (2 g/cm(2)) for various time durations (0.5, 2, 6, 24, 48 hrs) with or without a knockdown treatment for FAK. Compressive stimulation increased the level of phosphorylated FAK and prostaglandin E(2) production. The FAK-knockdown cells showed significantly lower prostaglandin E(2) levels than the control cells. Furthermore, compressive stress up-regulated cyclo-oxygenase-2 mRNA, whereas there were no changes observed in the FAK-knockdown cells. These results suggest that FAK regulates the production of prostaglandin E(2) via the transcriptional regulation of COX-2 mRNA in compressive stimulated PDL cells. The FAK-integrin complex plays a role in mechanoreception and mechanotransduction in hPDL cells.
- Kyung Hee University Korea (Republic of)
Time Factors, Transcription, Genetic, Cell Survival, Periodontal Ligament, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Mechanotransduction, Cellular, Dinoprostone, Biomechanical Phenomena, Cyclooxygenase 2, Focal Adhesion Kinase 1, Gene Knockdown Techniques, Humans, Electrophoresis, Polyacrylamide Gel, Stress, Mechanical, Phosphorylation, RNA, Small Interfering, Mechanoreceptors, Protein Processing, Post-Translational, Cells, Cultured
Time Factors, Transcription, Genetic, Cell Survival, Periodontal Ligament, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Mechanotransduction, Cellular, Dinoprostone, Biomechanical Phenomena, Cyclooxygenase 2, Focal Adhesion Kinase 1, Gene Knockdown Techniques, Humans, Electrophoresis, Polyacrylamide Gel, Stress, Mechanical, Phosphorylation, RNA, Small Interfering, Mechanoreceptors, Protein Processing, Post-Translational, Cells, Cultured
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