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Subtractive cloning of a hybrid human endogenous retrovirus and calbindin gene in the prostate cell line PC3.

Authors: A Y, Liu; B A, Abraham;

Subtractive cloning of a hybrid human endogenous retrovirus and calbindin gene in the prostate cell line PC3.

Abstract

A complementary DNA clone containing human endogenous retrovirus-like sequences spliced to sequences encoding human calbindin was discovered by complementary DNA subtraction analysis between two human prostate cell lines, PC3 and DU145. This gene is presumably activated by the long terminal repeat of the retrovirus-like sequence. It belongs to a member of the retrovirus-like H (tRNA(his) primer binding sequence) family. The level of this transcript is high in PC3, derived from a prostate bone metastasis, but not in DU145, derived from a prostate brain metastasis.

Keywords

Male, Calbindins, Base Sequence, Transcription, Genetic, Molecular Sequence Data, Retinoblastoma, Prostatic Neoplasms, DNA, Middle Aged, Retroviridae, S100 Calcium Binding Protein G, Tumor Cells, Cultured, Humans, RNA, RNA, Messenger, Cloning, Molecular, Poly A, Aged

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Powered by OpenAIRE graph
citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
55
Average
Top 10%
Top 10%