Genotyping for RhC/c and RhE/e by PCR using allele-specific oligonucleotide primers.
Copyright policy )Genotyping for RhC/c and RhE/e by PCR using allele-specific oligonucleotide primers.
The Rh blood group system has five major antigens D, C/c, and E/e. These antigens are encoded in RHD and RHCE genes. In this report, we describe a systemic method for RhC/c and RhE/e genotyping by PCR using allele-specific oligonucleotide primers (ASO-PCR). The ASO-PCR was carried out to determine the RhC/c and RhE/e genotypes in DNA samples from 513 Japanese donors. Genotypes of RhC, RhE, and Rhe were in full concordance with serological phenotypes in 511 donors. However, in two cases with the phenotype of ccdee, the C-specific ASO-PCR product was also detected in addition to the c-specific one. This method is simple and quite useful for the RhC/c and RhE/c genotyping, although further investigation on the 2 exceptional ccdee cases is needed.
Isoantigens, Rh-Hr Blood-Group System, Base Sequence, Genotype, Molecular Sequence Data, Humans, Polymerase Chain Reaction, DNA Primers
Isoantigens, Rh-Hr Blood-Group System, Base Sequence, Genotype, Molecular Sequence Data, Humans, Polymerase Chain Reaction, DNA Primers
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).6 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!