[The mutation and expression of MUS81 gene in laryngeal squamous cell carcinoma].
Copyright policy )[The mutation and expression of MUS81 gene in laryngeal squamous cell carcinoma].
To investigate the association of mutations and expression of MUS81 gene with the tumorigenesis and progression of laryngeal squamous cell carcinoma (LSCC).PCR-SSCP and DNA sequencing were carried out to examine mutations at exons 9 and 10 of MUS81 gene in 42 LSCC samples, with paired adjacent normal laryngeal tissues (PANLs) as control. Semi-quantitative RT-PCR and Western blot were used to detect the expression of MUS81 gene in the specimens.No mutation was detected in the control group. Among the 42 LSCC specimens, nineteen (45.2%) were found to harbor mutations, including 11(26.2%) occurring within exon 9, and 8 (19%) within exon 10. Seventeen (40.48%) samples showed lower mRNA level of the MUS81 gene (P0.05). Nor did the expression of MUS81 gene with the TNM stages, age and lymphoid node metastasis in LSCC (P>0.05).Mutations and abnormal expression of MUS81 gene in the LSCC tissues were observed, which suggested that abnormalities of MUS81 gene may play an important role in the tumorigenesis of LSCC.
- China Medical University China (People's Republic of)
Base Sequence, Molecular Sequence Data, Age Factors, Exons, Endonucleases, DNA-Binding Proteins, Gene Expression Regulation, Neoplastic, Lymphatic Metastasis, Mutation, Carcinoma, Squamous Cell, Humans, RNA, Messenger, Laryngeal Neoplasms, Neoplasm Staging
Base Sequence, Molecular Sequence Data, Age Factors, Exons, Endonucleases, DNA-Binding Proteins, Gene Expression Regulation, Neoplastic, Lymphatic Metastasis, Mutation, Carcinoma, Squamous Cell, Humans, RNA, Messenger, Laryngeal Neoplasms, Neoplasm Staging
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