To investigate the sequence features of FUT2/01 locus and its polymorphic distribution in Chinese population, and to discuss its application potential in forensic medicine.The alleles on FUT2/01 locus were amplified by PCR and then were sequenced. Furthermore, polymorphic distribution of the locus was analyzed by polyacrylamide gel electrophoresis. The genotypes were characterized with fluorescence labeling followed by automatic detection system.The sequencing results only showed the length differences which were determined by the tandem repeats variance of the core sequence. There were 9 alleles and 28 genotypes identified from 162 individuals. The discrimination power and excluding probability of paternity were 0.9639 and 0.6266, respectively. In addition, the locus could be genotyped by automatic analysis very well.The FUT2/01 locus exhibits high heterozygosity and individual identification power in Chinese Han population, and may be a valuable STR system for application in forensic medicine.