Viral protein U counteracts a human host cell restriction that inhibits HIV-1 particle production
Copyright policy )Viral protein U counteracts a human host cell restriction that inhibits HIV-1 particle production
Human cells resist viral infections by a variety of mechanisms. Viruses must overcome host cell restrictions to successfully reproduce their genetic material. Here, we identify a host restriction to viral replication that acts at the stage of particle assembly. Viral protein U (Vpu) is an HIV-1 accessory protein that enhances particle assembly and release in most human cells, but not in simian cells. By using human-simian cell heterokaryons, we show that the inhibition of assembly in human cells is dominant. Vpu overcomes the block to assembly in human cells and in human-simian heterokaryons. The HIV-1 vpu gene may have evolved to counteract an assembly restriction that is present in human cells.
- Vanderbilt University United States
- National Institutes of Health United States
- National Institute of Allergy and Infectious Diseases United States
Time Factors, Human Immunodeficiency Virus Proteins, Gene Products, gag, Transfection, Virus Replication, Cell Line, Polyethylene Glycols, Cell Fusion, Viral Proteins, Phenotype, COS Cells, Chlorocebus aethiops, HIV-1, Animals, Humans, Viral Regulatory and Accessory Proteins, Vero Cells, HeLa Cells, Plasmids
Time Factors, Human Immunodeficiency Virus Proteins, Gene Products, gag, Transfection, Virus Replication, Cell Line, Polyethylene Glycols, Cell Fusion, Viral Proteins, Phenotype, COS Cells, Chlorocebus aethiops, HIV-1, Animals, Humans, Viral Regulatory and Accessory Proteins, Vero Cells, HeLa Cells, Plasmids
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