7SK small nuclear ribonucleoprotein complex is recruited to the HIV‐1 promoter via short viral transcripts
Copyright policy )7SK small nuclear ribonucleoprotein complex is recruited to the HIV‐1 promoter via short viral transcripts
In this study, we demonstrate that the 7SK small nuclear ribonucleoprotein (snRNP) complex is recruited to the HIV‐1 promoter via newly‐synthesized HIV‐1 nascent transcripts (short transcripts) in an hnRNP A1‐dependent manner and negatively regulates viral transcript elongation. Our deep‐sequence analysis showed these short transcripts were mainly arrested at approximately +50 to +70 nucleotides from the transcriptional start site in the U1 cells, an HIV‐1 latent model. TNF‐α treatment promptly disrupted the 7SK snRNP complex on the nascent transcripts and viral elongated transcripts were increased. This report provides insight into how 7SK snRNP complex is recruited to HIV‐1 promoter in the absence of Tat.
- Japan Science and Technology Agency Japan
- University of Tokyo Japan
- National Presto Industries United States
Gene Expression Regulation, Viral, RNA virus, Molecular biology, Heterogeneous Nuclear Ribonucleoprotein A1, Molecular Sequence Data, Viral transcription, Virology, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Humans, RNA, Small Interfering, Promoter Regions, Genetic, Chromatin regulation, Base Sequence, Tumor Necrosis Factor-alpha, Inverted Repeat Sequences, RNA-Binding Proteins, HEK293 Cells, Ribonucleoproteins, Gene Knockdown Techniques, HIV-1, RNA, Viral, Transcription Factors
Gene Expression Regulation, Viral, RNA virus, Molecular biology, Heterogeneous Nuclear Ribonucleoprotein A1, Molecular Sequence Data, Viral transcription, Virology, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Humans, RNA, Small Interfering, Promoter Regions, Genetic, Chromatin regulation, Base Sequence, Tumor Necrosis Factor-alpha, Inverted Repeat Sequences, RNA-Binding Proteins, HEK293 Cells, Ribonucleoproteins, Gene Knockdown Techniques, HIV-1, RNA, Viral, Transcription Factors
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