Characterization of SVEP1, KIAA, and SRPX2 in an in vitro cell culture model of endotoxemia
Copyright policy )Characterization of SVEP1, KIAA, and SRPX2 in an in vitro cell culture model of endotoxemia
To assess the influence of unknown factors in endotoxemia, a conditioned medium, achieved by the stimulation of THP1 monocytes with lipopolysaccharide (LPS) [4h], was used for the stimulation of human umbilical vein endothelial cells (HUVECs) [16h]. SVEP1, KIAA0247, and SRPX2 were selected after microarray analysis. To study their possible functions, siRNAs of SVEP1, KIAA0247, or SRPX2 were used for the transfection of HUVECs and cells were stimulated with conditioned medium [16h]. Inhibition of SVEP1 expression resulted in an increase of soluble intercellular adhesion molecule (sICAM) 1 (10%) and soluble E-selectin (sE-selectin) (19%). Inhibition of SRPX2 led to an increase of sICAM (11%) and sE-selectin (14%). KIAA0247 negative HUVECs showed a decrease in monocyte chemoattractant protein (MCP) 1 of 16%. SVEP1 and SRPX2 seemed to act as regulators of ICAM1 and E-selectin shedding and influence the expression of membrane bound adhesion molecules.
- Danube University Krems Austria
Inflammation, Lipopolysaccharides, Endothelial Cells, Membrane Proteins, Nerve Tissue Proteins, Complement System Proteins, Microarray Analysis, Endotoxemia, Monocytes, Cell Line, Neoplasm Proteins, Gene Expression Regulation, Culture Media, Conditioned, Cytokines, Humans, L-Selectin, RNA, Small Interfering, Cell Adhesion Molecules
Inflammation, Lipopolysaccharides, Endothelial Cells, Membrane Proteins, Nerve Tissue Proteins, Complement System Proteins, Microarray Analysis, Endotoxemia, Monocytes, Cell Line, Neoplasm Proteins, Gene Expression Regulation, Culture Media, Conditioned, Cytokines, Humans, L-Selectin, RNA, Small Interfering, Cell Adhesion Molecules
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