FNDC5 induces M2 macrophage polarization and promotes hepatocellular carcinoma cell growth by affecting the PPARγ/NF-κB/NLRP3 pathway
Copyright policy )FNDC5 induces M2 macrophage polarization and promotes hepatocellular carcinoma cell growth by affecting the PPARγ/NF-κB/NLRP3 pathway
The purpose of this study was to investigate the effect of FNDC5 expression levels in hepatocellular carcinoma on the phenotypic changes of macrophages in tumor tissues.In this study, we established an in vitro co-culture system of hepatocellular carcinoma cells and macrophages. Then we performed overexpression or knockdown of FNDC5 gene in hepatocellular carcinoma cells to observe the effect of changes in FNDC5 expression level on the phenotypic changes of THP-1 macrophages. And the conclusions obtained in the in vitro assay were further validated by a subcutaneous tumorigenic nude mice model.Our findings suggest that elevated FNDC5 expression in hepatocellular carcinoma cells lead to an increased M2 phenotype and decreased M1 phenotype in macrophages. This effect may be achieved by elevating PPARγ levels in macrophages while decreasing NF-κB and NLRP3 levels. These changes could be reversed by using PPARγ inhibitors.We preliminarily demonstrated that FNDC5 in hepatocellular carcinoma cells promotes the polarization of M2 macrophages by affecting the PPARγ/NF-κB/NLRP3 pathway.
- Qingdao Binhai University China (People's Republic of)
- Dalian Medical University China (People's Republic of)
- Qingdao University China (People's Republic of)
Carcinoma, Hepatocellular, Carcinogenesis, Liver Neoplasms, NF-kappa B, Mice, Nude, Cell Differentiation, Hep G2 Cells, Survival Analysis, Coculture Techniques, Fibronectins, Gene Expression Regulation, Neoplastic, PPAR gamma, Mice, Cell Line, Tumor, NLR Family, Pyrin Domain-Containing 3 Protein, Animals, Humans, Anilides, RNA, Small Interfering, Signal Transduction
Carcinoma, Hepatocellular, Carcinogenesis, Liver Neoplasms, NF-kappa B, Mice, Nude, Cell Differentiation, Hep G2 Cells, Survival Analysis, Coculture Techniques, Fibronectins, Gene Expression Regulation, Neoplastic, PPAR gamma, Mice, Cell Line, Tumor, NLR Family, Pyrin Domain-Containing 3 Protein, Animals, Humans, Anilides, RNA, Small Interfering, Signal Transduction
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