Gap junctions mediate STAT5-independent β-casein expression in CID-9 mammary epithelial cells
Copyright policy )Gap junctions mediate STAT5-independent β-casein expression in CID-9 mammary epithelial cells
Crosstalk between gap junction intracellular communication (GJIC), STAT5 and OCT-1 in gap junction (GJ)-dependent β-casein expression was investigated. CID-9 mammary cells plated with prolactin on non-adherent substratum (poly-HEMA) expressed β-casein independent of STAT5 only in the presence of the GJIC inducer, cAMP. Nuclear STAT5 levels were not detectable. By contrast, cells on EHS-drip expressed β-casein in a STAT5-dependent manner and nuclear STAT5 levels were up-regulated. A 75 kDa OCT-1 isoform was detected in conditions that induced β-casein expression regardless of substratum. Interestingly, 40 and 28 kDa OCT-1 isoforms were induced in cells on polyHEMA with cAMP. Electrophoretic mobility shift assays (EMSA) for OCT-1 revealed two band shifts in cells on polyHEMA with cAMP and on EHS-drip, which were repressed by the GJIC inhibitor, 18α-GA. These studies demonstrated that mammary cells on polyHEMA expressed β-casein in response to prolactin in a pathway that involves GJIC and OCT-1 and is independent of STAT5 nuclear translocation.
- American University of Beirut Lebanon
Active Transport, Cell Nucleus, Cell Culture Techniques, Caseins, Gap Junctions, Gene Expression, Cell Differentiation, Epithelial Cells, Receptor Cross-Talk, Janus Kinase 2, Cell Line, Prolactin, Mice, Mammary Glands, Animal, Gene Expression Regulation, Animals, Female, Phosphorylation, DNA Probes, Octamer Transcription Factor-1, Protein Binding
Active Transport, Cell Nucleus, Cell Culture Techniques, Caseins, Gap Junctions, Gene Expression, Cell Differentiation, Epithelial Cells, Receptor Cross-Talk, Janus Kinase 2, Cell Line, Prolactin, Mice, Mammary Glands, Animal, Gene Expression Regulation, Animals, Female, Phosphorylation, DNA Probes, Octamer Transcription Factor-1, Protein Binding
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