A Combined Computational and Experimental Study on the Structure-Regulation Relationships of Putative Mammalian DNA Replication Initiator GINS
Copyright policy )A Combined Computational and Experimental Study on the Structure-Regulation Relationships of Putative Mammalian DNA Replication Initiator GINS
Abstract GINS, a heterotetramer of SLD5, PSF1, PSF2, and PSF3 proteins, is an emerging chromatin factor recognized to be involved in the initiation and elongation step of DNA replication. Although the yeast and Xenopus GINS genes are well documented, their orthologous genes in higher eukaryotes are not fully characterized. In this study, we report the genomic structure and transcriptional regulation of mammalian GINS genes. Serum stimulation increased the GINS mRNA levels in human cells. Reporter gene assay using putative GINS promoter sequences revealed that the expression of mammalian GINS is regulated by 17β-Estradiol-stimulated estrogen receptor α, and human PSF3 acts as a gene responsive to transcription factor E2F1. The goal of this study is to present the current data so as to encourage further work in the field of GINS gene regulation and functions in mammalian cells.
- Meiji University Japan
- Meijo University Japan
Transcription, Genetic, Biochemistry, Article, Mice, Structure-Activity Relationship, Genetics, Animals, Humans, Tissue Distribution, Molecular Biology, DNA replication initiation, Estradiol, DNA Helicases, Estrogen Receptor alpha, Computational Biology, bioinformatics, DNA, gene structure, transcription factor E2F1, Chromatin, 17β-Estradiol, DNA-Binding Proteins, Computational Mathematics, Gene Expression Regulation, Trans-Activators, gene regulation, E2F1 Transcription Factor, HeLa Cells
Transcription, Genetic, Biochemistry, Article, Mice, Structure-Activity Relationship, Genetics, Animals, Humans, Tissue Distribution, Molecular Biology, DNA replication initiation, Estradiol, DNA Helicases, Estrogen Receptor alpha, Computational Biology, bioinformatics, DNA, gene structure, transcription factor E2F1, Chromatin, 17β-Estradiol, DNA-Binding Proteins, Computational Mathematics, Gene Expression Regulation, Trans-Activators, gene regulation, E2F1 Transcription Factor, HeLa Cells
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