Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of initiation factor 1 fromMycobacterium tuberculosis
Copyright policy )Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of initiation factor 1 fromMycobacterium tuberculosis
Initiation factor 1 (IF-1; Rv3462c) from Mycobacterium tuberculosis, a component of the 30S initiation complex, was cloned and heterologously expressed in Escherichia coli. The protein was purified by affinity and size-exclusion chromatography and crystallized. A complete data set has been collected to high resolution. The crystals belonged to space group P2(1)2(1)2, with two molecules per asymmetric unit which are related by translational symmetry.
- European Bioinformatics Institute United Kingdom
- European Molecular Biology Laboratory Germany
Molecular Weight, Bacterial Proteins, Prokaryotic Initiation Factor-1, Gene Expression Regulation, Bacterial, Mycobacterium tuberculosis, Cloning, Molecular, Crystallization, Crystallography, X-Ray
Molecular Weight, Bacterial Proteins, Prokaryotic Initiation Factor-1, Gene Expression Regulation, Bacterial, Mycobacterium tuberculosis, Cloning, Molecular, Crystallization, Crystallography, X-Ray
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