Promoter Methylation of TSLC1 and Tumor Suppression by Its Gene Product in Human Prostate Cancer
Copyright policy )Promoter Methylation of TSLC1 and Tumor Suppression by Its Gene Product in Human Prostate Cancer
We recently identified TSLC1, a tumor suppressor gene in human lung cancer. Gene silencing by promoter methylation has been observed frequently in adenocarcinoma of the lung, liver, and pancreas. Here, we demonstrate that TSLC1 expression is also absent or markedly reduced in 3 of 4 prostate cancer cell lines. Promoter sequences of TSLC1 were heavily methylated in PPC‐1 cells that lacked TSLC1 expression, supporting the idea that promoter methylation is strongly correlated with complete loss of gene expression. Promoter sequences of TSLC1 were also methylated significantly in 7 of 22 (32%) primary prostate cancers. Hypermethylation of the promoter occurred not only in advanced tumors, but also in relatively early‐stage tumors. Restoration of TSLC1 expression substantially suppressed tumor formation of PPC‐1 cells in nude mice. These findings indicate that alteration of TSLC1 is involved in prostate cancer.
- Johns Hopkins University School of Medicine United States
- National Cancer Research Institute United Kingdom
- Kōchi University Japan
- University of Tokyo Japan
- Johns Hopkins University Sch of Medicine United States
Male, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Molecular Sequence Data, Cell Adhesion Molecule-1, Immunoglobulins, Membrane Proteins, Mice, Nude, Prostatic Neoplasms, Proteins, DNA Methylation, Blotting, Northern, Mice, Animals, Humans, Sulfites, CpG Islands, Gene Silencing, Promoter Regions, Genetic, Cell Adhesion Molecules, Rapid Communication
Male, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Molecular Sequence Data, Cell Adhesion Molecule-1, Immunoglobulins, Membrane Proteins, Mice, Nude, Prostatic Neoplasms, Proteins, DNA Methylation, Blotting, Northern, Mice, Animals, Humans, Sulfites, CpG Islands, Gene Silencing, Promoter Regions, Genetic, Cell Adhesion Molecules, Rapid Communication
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