Ermap, a gene coding for a novel erythroid specific adhesion/receptor membrane protein
Copyright policy )Ermap, a gene coding for a novel erythroid specific adhesion/receptor membrane protein
Ermap (erythroid membrane-associated protein), a gene coding for a novel transmembrane protein produced exclusively in erythroid cells, is described. It is mapped to murine Chromosome 4, 57 cM distal to the centromere. The initial cDNA clone was isolated from a day 9 murine embryonic erythroid cell cDNA library. The predicted peptide sequence suggests that ERMAP is a transmembrane protein with two extracellular immunoglobulin folds, as well as a highly conserved B30.2 domain and several phosphorylation consensus sequences in the cytoplasmic region. ERMAP shares a high homology throughout the entire peptide with butyrophilin, a glycoprotein essential for milk lipid droplet formation and release. A GFP-ERMAP fusion protein was localized to the plasma membrane and cytoplasmic vesicles in transiently transfected 293T cells. Northern blot analysis and in-situ hybridization demonstrated that Ermap expression was restricted to fetal and adult erythroid tissues. ERMAP is likely a novel adhesion/receptor molecule specific for erythroid cells.
- Monash University Australia
- University of Queensland Australia
- McMaster University Canada
- Boston Children's Hospital United States
- Harvard University United States
Male, Cytoplasm, Luminescent-Proteins, Erythrocytes, Mouse, Transcription Factor, Blotting-Northern, Immunoglobulin Superfamily, In-Situ-Hybridization, Gene-Expression-Regulation-Developmental, Major Histocompatibility Complex, Mice, Tissue-Distribution, SUPPORT-U-S-GOVT-P-H-S, Membrane-Proteins, Butyrophilin, Cloning, Molecular, In Situ Hybridization, Chromosome Mapping, Gene Expression Regulation, Developmental, Sequence-Homology-Amino-Acid, Fat Globule-Membrane, Cell-Line, Cloning-Molecular, Embryo, Blood Group Antigens, K562-Cells, Sequence-Analysis-DNA, Cell-Adhesion-Molecules, Gene-Expression-Regulation, DNA-Complementary, Human, Cell-Adhesion, 570, DNA, Complementary, Green Fluorescent Proteins, Recombinant-Fusion-Proteins, 610, Sequence-Alignment, Cell Line, Sequence, Cell Adhesion, Animals, Humans, Amino Acid Sequence, Cell-Nucleus, SUPPORT-NON-U-S-GOVT, Cell Nucleus, Molecular-Sequence-Data, Butyrophilins, Animal, Macrophages, Mice-Inbred-C57BL, Blotting, Northern, Embryo, Mammalian, Muridae, Gene Expression Regulation, Chromosome-Mapping, RNA, Mammary Epithelial-Cells, Glycoprotein, Cell Adhesion Molecules
Male, Cytoplasm, Luminescent-Proteins, Erythrocytes, Mouse, Transcription Factor, Blotting-Northern, Immunoglobulin Superfamily, In-Situ-Hybridization, Gene-Expression-Regulation-Developmental, Major Histocompatibility Complex, Mice, Tissue-Distribution, SUPPORT-U-S-GOVT-P-H-S, Membrane-Proteins, Butyrophilin, Cloning, Molecular, In Situ Hybridization, Chromosome Mapping, Gene Expression Regulation, Developmental, Sequence-Homology-Amino-Acid, Fat Globule-Membrane, Cell-Line, Cloning-Molecular, Embryo, Blood Group Antigens, K562-Cells, Sequence-Analysis-DNA, Cell-Adhesion-Molecules, Gene-Expression-Regulation, DNA-Complementary, Human, Cell-Adhesion, 570, DNA, Complementary, Green Fluorescent Proteins, Recombinant-Fusion-Proteins, 610, Sequence-Alignment, Cell Line, Sequence, Cell Adhesion, Animals, Humans, Amino Acid Sequence, Cell-Nucleus, SUPPORT-NON-U-S-GOVT, Cell Nucleus, Molecular-Sequence-Data, Butyrophilins, Animal, Macrophages, Mice-Inbred-C57BL, Blotting, Northern, Embryo, Mammalian, Muridae, Gene Expression Regulation, Chromosome-Mapping, RNA, Mammary Epithelial-Cells, Glycoprotein, Cell Adhesion Molecules
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