Calbindin D 9k knockout mice are indistinguishable from wild-type mice in phenotype and serum calcium level
Copyright policy )Calbindin D 9k knockout mice are indistinguishable from wild-type mice in phenotype and serum calcium level
Since the discovery of calbindin D 9k , its role in intestinal calcium absorption has remained unsettled. Further, a wide distribution of calbindin D 9k among tissues has argued for its biological importance. We discovered a frameshift deletion in the calbindin D 9k gene in an ES cell line, E14.1, that originated from 129/OlaHsd mice. We produced mice with the mutant calbindin D 9k gene by injecting the E14.1 ES cell subline into the C57BL/6 host blastocysts and proved that these mice lack calbindin D 9k protein. Calbindin D 9k knockout mice were indistinguishable from wild-type mice in phenotype, were able to reproduce, and had normal serum calcium levels. Thus, calbindin D 9k is not required for viability, reproduction, or calcium homeostasis.
- University of Wisconsin–Madison United States
- University of North Carolina at Chapel Hill United States
- Rutgers New Jersey Medical School United States
- University of Wisconsin–Oshkosh United States
- LFB (France) France
Mice, Knockout, Calbindins, Base Sequence, Molecular Sequence Data, Mice, Inbred Strains, Cell Line, Mice, Phenotype, S100 Calcium Binding Protein G, Animals, Calcium, Frameshift Mutation
Mice, Knockout, Calbindins, Base Sequence, Molecular Sequence Data, Mice, Inbred Strains, Cell Line, Mice, Phenotype, S100 Calcium Binding Protein G, Animals, Calcium, Frameshift Mutation
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