Molecular-Cloning and Tissue-Specific Expression of Fructose 6-Phosphate 2-Kinase:Fructose 2,6-Bisphosphatase of Rat Brain
Copyright policy )Molecular-Cloning and Tissue-Specific Expression of Fructose 6-Phosphate 2-Kinase:Fructose 2,6-Bisphosphatase of Rat Brain
A cDNA clone (3591 base pairs) encoding an isozyme of Fructose 6-phosphate, 2-kinase:Fructose 2,6-bisphosphatase was isolated from a rat brain cDNA library. The 5' sequence of this clone (1241 base pairs) was identical to that of the heart type isozyme cDNA (except for 7 base pair mismatches), but the 3' nucleotide sequence (2024 base pairs) was completely different. Its deduced amino acid sequence showed that the enzyme lacked a regulatory domain which contained phosphorylation sites for protein kinase A and C. The results of Northern blotting and polymerase chain reaction demonstrated that the mRNA, 7.4 kilobases long, was expressed also in heart, testis, liver, and skeletal muscle.
- United States Department of Veterans Affairs United States
- The University of Texas at Dallas United States
Male, Base Sequence, Phosphofructokinase-2, Muscles, Myocardium, Molecular Sequence Data, Brain, Gene Expression, Blotting, Northern, Isoenzymes, Molecular Weight, Alternative Splicing, Liver, Multienzyme Complexes, Organ Specificity, Animals, Amino Acid Sequence, Cloning, Molecular, DNA Primers, Gene Library
Male, Base Sequence, Phosphofructokinase-2, Muscles, Myocardium, Molecular Sequence Data, Brain, Gene Expression, Blotting, Northern, Isoenzymes, Molecular Weight, Alternative Splicing, Liver, Multienzyme Complexes, Organ Specificity, Animals, Amino Acid Sequence, Cloning, Molecular, DNA Primers, Gene Library
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