Phosphoregulation of MgcRacGAP in mitosis involves Aurora B and Cdk1 protein kinases and the PP2A phosphatase
Copyright policy )Phosphoregulation of MgcRacGAP in mitosis involves Aurora B and Cdk1 protein kinases and the PP2A phosphatase
MgcRacGAP, a Rho GAP essential to cytokinesis, works both as a Rho GTPase regulator and as a scaffolding protein. MgcRacGAP interacts with MKLP1 to form the centralspindlin complex and associates with the RhoGEF Ect2. The GAP activity of MgcRacGAP is regulated by Aurora B phosphorylation. We have isolated B56epsilon, a PP2A regulatory subunit, as a new MgcRacGAP partner. We report here that (i) MgcRacGAP is phosphorylated by Aurora B and Cdk1, (ii) PP2A dephosphorylates Aurora B and Cdk1 phosphorylated sites and (iii) inhibition of PP2A abrogates MgcRacGAP/Ect2 interaction. Therefore, PP2A may regulate cytokinesis by dephosphorylating MgcRacGAP and its interacting partners.
- Inserm France
- National Chiao Tung University Taiwan
- University of Paris France
- French Institute of Health and Medical Research France
- Institut Cochin France
GTPase-Activating Proteins, Mitosis, Protein Serine-Threonine Kinases, Aurora Kinases, Tandem Mass Spectrometry, Cell Line, Tumor, Two-Hybrid System Techniques, CDC2 Protein Kinase, COS Cells, Chlorocebus aethiops, RhoGAP, Animals, Aurora Kinase B, Humans, Protein Phosphatase 2, Phosphorylation, Phosphoprotein Phosphatase 2A, Chromatography, High Pressure Liquid, Cytokinesis
GTPase-Activating Proteins, Mitosis, Protein Serine-Threonine Kinases, Aurora Kinases, Tandem Mass Spectrometry, Cell Line, Tumor, Two-Hybrid System Techniques, CDC2 Protein Kinase, COS Cells, Chlorocebus aethiops, RhoGAP, Animals, Aurora Kinase B, Humans, Protein Phosphatase 2, Phosphorylation, Phosphoprotein Phosphatase 2A, Chromatography, High Pressure Liquid, Cytokinesis
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