Genomic cloning, structure, and regulatory elements of the 1α,25(OH)2D3 down-regulated gene for cyclic AMP-dependent protein kinase inhibitor
Copyright policy )Genomic cloning, structure, and regulatory elements of the 1α,25(OH)2D3 down-regulated gene for cyclic AMP-dependent protein kinase inhibitor
The cyclic AMP-dependent protein kinase inhibitor (PKI) mRNA and protein are negatively and tissue-specifically regulated in the kidney by 1 alpha, 25(OH)2D3. A 17-kb PKI clone, isolated from a chick genomic library, revealed that the PKI gene consists of two exons separated by a 4.5-kb intron. A 411-bp upstream region (constituting 93 bp upstream and 318 bp downstream from the transcriptional start site) containing a putative negative VDRE (nVDRE) fused to the luciferase gene was used for transient transfections of primary cultures of chick kidney cells. Luciferase activity was significantly down-regulated in response to 1 alpha, 25(OH)2D3. This result suggests that the promoter region containing the putative nVDRE plays a pivotal role in the negative regulation of PKI gene transcription.
- University of California, Irvine United States
- University of California System United States
- University of California, Riverside United States
DNA, Complementary, Base Sequence, Molecular Sequence Data, Intracellular Signaling Peptides and Proteins, Down-Regulation, Regulatory Sequences, Nucleic Acid, Kidney, Transfection, beta-Galactosidase, Calcitriol, Gene Expression Regulation, Animals, Amino Acid Sequence, Cloning, Molecular, Carrier Proteins, Luciferases, Promoter Regions, Genetic, Chickens, Cells, Cultured
DNA, Complementary, Base Sequence, Molecular Sequence Data, Intracellular Signaling Peptides and Proteins, Down-Regulation, Regulatory Sequences, Nucleic Acid, Kidney, Transfection, beta-Galactosidase, Calcitriol, Gene Expression Regulation, Animals, Amino Acid Sequence, Cloning, Molecular, Carrier Proteins, Luciferases, Promoter Regions, Genetic, Chickens, Cells, Cultured
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