AbstractWe propose two models of the human S‐arrestin/rhodopsin complex in the inactive dark adapted rhodopsin and meta rhodopsin II form, obtained by homology modeling and knowledge based docking. First, a homology model for the human S‐arrestin was built and validated by molecular dynamics, showing an average root mean square deviation difference from the pattern behavior of 0.76 Å. Then, combining the human S‐arrestin model and the modeled structure of the two human rhodopsin forms, we propose two models of interaction for the human S‐arrestin/rhodopsin complex. The models involve two S‐arrestin regions related to the N domain (residues 68–78; 170–182) and a third constituent of the C domain (248–253), with the rhodopsin C terminus (330–348). Of the 22 single point mutations related to retinitis pigmentosa and congenital night blindness located in the cytoplasmatic portion of rhodopsin or in S‐arrestin, our models locate 16 in the interaction region and relate two others to possible dimer formation. Our calculations also predict that the light activated complex is more stable than the dark adapted rhodopsin and, therefore, of higher affinity to S‐arrestin. Proteins 2008. © 2008 Wiley‐Liss, Inc.