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Proceedings of the National Academy of Sciences
Article . 2006 . Peer-reviewed
Data sources: Crossref
UNC Dataverse
Article . 2006
Data sources: Datacite
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Genetic mosaics reveal both cell-autonomous and cell-nonautonomous function of murine p27 Kip1

Authors: Marcelo L. Rodriguez-Puebla; Kendra Garrison; Jason Orthel; Wei-Ming Chien; Matthew L. Fero; Everardo Macias; Stuart Rabin; +1 Authors

Genetic mosaics reveal both cell-autonomous and cell-nonautonomous function of murine p27 Kip1

Abstract

Loss of the cyclin-dependent kinase inhibitor p27 Kip1 leads to an overall increase in animal growth, pituitary tumors, and hyperplasia of hematopoietic organs, yet it is unknown whether all cells function autonomously in response to p27 Kip1 activity or whether certain cells take cues from their neighbors. In addition, there is currently no genetic evidence that tumor suppression by p27 Kip1 is cell-autonomous because biallelic gene inactivation is absent from tumors arising in p27 Kip1 hemizygous mice. We have addressed these questions with tissue-specific targeted mouse mutants and radiation chimeras. Our results indicate that the suppression of pars intermedia pituitary tumors by p27 Kip1 is cell-autonomous and does not contribute to overgrowth or infertility phenotypes. In contrast, suppression of spleen growth and hematopoietic progenitor expansion is a consequence of p27 Kip1 function external to the hematopoietic compartment. Likewise, p27 Kip1 suppresses thymocyte hyperplasia through a cell-nonautonomous mechanism. The interaction of p27 Kip1 loss with epithelial cell-specific cyclin-dependent kinase 4 overexpression identifies the thymic epithelium as a relevant site of p27 Kip1 activity for the regulation of thymus growth.

Keywords

Male, Mice, Knockout, Hyperplasia, Base Sequence, Mosaicism, Mice, Transgenic, DNA, Thymus Gland, Mice, Pituitary Gland, Radiation Chimera, Mutation, Animals, Female, Pituitary Neoplasms, Cyclin-Dependent Kinase Inhibitor p27, Spleen

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    38
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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    influence
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    impulse
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    Top 10%
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
38
Average
Top 10%
Top 10%
bronze