Exploring the Interactome: Microfluidic Isolation of Proteins and Interacting Partners for Quantitative Analysis by Electron Microscopy
pmid: 24773394
Exploring the Interactome: Microfluidic Isolation of Proteins and Interacting Partners for Quantitative Analysis by Electron Microscopy
Multimolecular protein complexes are important for many cellular processes. However, the stochastic nature of the cellular interactome makes the experimental detection of complex protein assemblies difficult and quantitative analysis at the single molecule level essential. Here, we present a fast and simple microfluidic method for (i) the quantitative isolation of endogenous levels of untagged protein complexes from minute volumes of cell lysates under close to physiological conditions and (ii) the labeling of specific components constituting these complexes. The method presented uses specific antibodies that are conjugated via a photocleavable linker to magnetic beads that are trapped in microcapillaries to immobilize the target proteins. Proteins are released by photocleavage, eluted, and subsequently analyzed by quantitative transmission electron microscopy at the single molecule level. Additionally, before photocleavage, immunogold can be employed to label proteins that interact with the primary target protein. Thus, the presented method provides a new way to study the interactome and, in combination with single molecule transmission electron microscopy, to structurally characterize the large, dynamic, heterogeneous multimolecular protein complexes formed.
- University of Zurich Switzerland
- University of Basel Switzerland
1602 Analytical Chemistry, SX20 Research, Technology and Development Projects, Microfluidics, Proteins, Cell Line, Magnetics, SX00 SystemsX.ch, Microscopy, Electron, Transmission, Cricetinae, Protein Interaction Mapping, SX03 CINA, 570 Life sciences; biology, Animals, Humans, Biotinylation
1602 Analytical Chemistry, SX20 Research, Technology and Development Projects, Microfluidics, Proteins, Cell Line, Magnetics, SX00 SystemsX.ch, Microscopy, Electron, Transmission, Cricetinae, Protein Interaction Mapping, SX03 CINA, 570 Life sciences; biology, Animals, Humans, Biotinylation
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