Structure of an RNA polymerase II preinitiation complex
Copyright policy )Structure of an RNA polymerase II preinitiation complex
Significance RNA polymerase II, the enzyme responsible for all mRNA synthesis in eukaryotes, requires a set of general transcription factors (GTFs) for the initiation of transcription. A complex of the polymerase and GTFs, with a mass of 1.5 MDa, was previously isolated and shown to be stable, homogeneous, and active in transcription. A cryo-electron microscope structure of the complex at a resolution of 6–11 Å reported here explains the requirement for the GTFs and elucidates their roles. The GTFs recognize the region of the gene responsible for initiation and deliver it to the polymerase active center in a form amenable to transcription.
- Hebrew University of Jerusalem Israel
- Stanford University United States
- University of Pennsylvania United States
- Scripps Research Institute United States
Models, Molecular, Transcription, Genetic, Cryoelectron Microscopy, DNA, Saccharomyces cerevisiae, TATA Box, Protein Structure, Tertiary, Protein Subunits, Transcription Factors, TFII, Multiprotein Complexes, Humans, Nucleic Acid Conformation, Protein Isoforms, RNA Polymerase II, Promoter Regions, Genetic, Protein Binding, Transcription Factors
Models, Molecular, Transcription, Genetic, Cryoelectron Microscopy, DNA, Saccharomyces cerevisiae, TATA Box, Protein Structure, Tertiary, Protein Subunits, Transcription Factors, TFII, Multiprotein Complexes, Humans, Nucleic Acid Conformation, Protein Isoforms, RNA Polymerase II, Promoter Regions, Genetic, Protein Binding, Transcription Factors
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