Distribution of Signaling Molecules Involved in Vasopressin-induced Ca2+Mobilization in Rat Hepatocyte Multiplets
Copyright policy )Distribution of Signaling Molecules Involved in Vasopressin-induced Ca2+Mobilization in Rat Hepatocyte Multiplets
In freshly isolated rat hepatocyte multiplets, Ca2+signals in response to vasopressin are highly organized. In this study we used specific probes to visualize, by fluorescence and confocal microscopy, the main signaling molecules involved in vasopressin-mediated Ca2+responses. V1a receptors were detected with a novel fluorescent antagonist, Rhm8-PVA. The Gαq/Gα11, PLCβ3, PIP2, and InsP3receptors were detected with specific antibodies. V1a vasopressin receptors and PIP2were associated with the basolateral membrane and were not detected in the bile canalicular domain. Gαq/Gα11, PLCβ3, and InsP3receptors were associated with the basolateral membrane and also with other intracellular structures. We used double labeling, Western blotting, and drugs (cytochalasin D, colchicine) known to disorganize the cytoskeleton to demonstrate the partial co-localization of Gαq/Gα11 with F-actin.
- University of Montpellier France
- University of Paris-Sud France
- University of Montpellier France
- University of Paris-Saclay France
Phosphatidylinositol 4,5-Diphosphate, Receptors, Vasopressin, Vasopressins, Phospholipase C beta, Inositol 1,4,5-Trisphosphate, Actins, Rats, Isoenzymes, Liver, Microscopy, Fluorescence, GTP-Binding Proteins, Tubulin, Type C Phospholipases, Animals, GTP-Binding Protein alpha Subunits, Gq-G11, Female, Calcium Signaling, Rats, Wistar, Cells, Cultured, Subcellular Fractions
Phosphatidylinositol 4,5-Diphosphate, Receptors, Vasopressin, Vasopressins, Phospholipase C beta, Inositol 1,4,5-Trisphosphate, Actins, Rats, Isoenzymes, Liver, Microscopy, Fluorescence, GTP-Binding Proteins, Tubulin, Type C Phospholipases, Animals, GTP-Binding Protein alpha Subunits, Gq-G11, Female, Calcium Signaling, Rats, Wistar, Cells, Cultured, Subcellular Fractions
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