Reduction of phosphodiesterase 3B gene expression in peroxisome proliferator‐activated receptor γ (+/−) mice independent of adipocyte size
Copyright policy )Reduction of phosphodiesterase 3B gene expression in peroxisome proliferator‐activated receptor γ (+/−) mice independent of adipocyte size
Phosphodiesterase 3B (PDE3B) gene expression is generally reduced in large adipocytes of obese, insulin‐resistant mice. This reduced gene expression is restored by peroxisome proliferator‐activated receptor (PPAR) γ ligands accompanied by a reduced fat cell size. To determine whether PDE3B gene expression is regulated by PPARγ itself, we analyzed lean PPARγ (+/−) mice with adipocyte size comparable to control PPARγ (+/+) mice. In adipocytes of PPARγ (+/−) mice, PDE3B mRNA and protein were both reduced to 63% of wild‐type levels. Basal PDE activity tended to be decreased to 70% of wild‐type levels, and, similarly, insulin‐induced PDE activity was significantly decreased to 70%. Thus, PPARγ is required for PDE3B gene expression independent of adipocyte size.
- University of Tokyo Japan
- University of Tokyo Japan
- Ehime University Japan
Blood Glucose, Male, Heterozygote, Receptors, Cytoplasmic and Nuclear, Peroxisome proliferator-activated receptor γ, Mice, Adipocytes, Animals, Insulin, RNA, Messenger, Epididymis, Adipocyte, Body Weight, Cell Membrane, Type 2 diabetes, Insulin resistance, Lipids, Cyclic Nucleotide Phosphodiesterases, Type 3, Adipose Tissue, Gene Expression Regulation, 3',5'-Cyclic-AMP Phosphodiesterases, Phosphodiesterase 3B, Transcription Factors
Blood Glucose, Male, Heterozygote, Receptors, Cytoplasmic and Nuclear, Peroxisome proliferator-activated receptor γ, Mice, Adipocytes, Animals, Insulin, RNA, Messenger, Epididymis, Adipocyte, Body Weight, Cell Membrane, Type 2 diabetes, Insulin resistance, Lipids, Cyclic Nucleotide Phosphodiesterases, Type 3, Adipose Tissue, Gene Expression Regulation, 3',5'-Cyclic-AMP Phosphodiesterases, Phosphodiesterase 3B, Transcription Factors
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