The Saccharomyces cerevisiae succinate‐ubiquinone reductase contains a stoichiometric amount of cytochrome b562
Copyright policy )The Saccharomyces cerevisiae succinate‐ubiquinone reductase contains a stoichiometric amount of cytochrome b562
The Saccharomyces cerevisiae succinate‐ubiquinone reductase or succinate dehydrogenase (SDH) is a tetramer of non‐equivalent subunits encoded by the SDH1, SDH2, SDH3, and SDH4 genes. In most organisms, SDH contains one or two endogenous b‐type hemes. However, it is widely believed that the yeast SDH does not contain heme. In this report, we demonstrate the presence of a stoichiometric amount of cytochrome b 562 in the yeast SDH. The cytochrome is detected as a peak present in fumarate‐oxidized, dithionite‐reduced mitochondria. The peak is centered at 562 nm and is present at a heme:covalent FAD molar ratio of 0.92±0.11. The cytochrome is not detectable in mitochondria isolated from SDH3 and SDH4 deletion strains. These observations strongly support our conclusion that cytochrome b 562 is a component of the yeast SDH.
- University of Alberta Canada
Heme, Saccharomyces cerevisiae, Fungal Proteins, Cytochrome b562, Mice, Fumarates, Multienzyme Complexes, Animals, Lactic Acid, Mitochondrion, Electron Transport Complex II, Escherichia coli Proteins, Spectrum Analysis, Dithionite, Intracellular Membranes, Cytochrome b Group, Yeast, Malonates, Mitochondria, Succinate dehydrogenase, Succinate Dehydrogenase, Covalent flavin, Flavin-Adenine Dinucleotide, Fumarate-oxidizable, Oxidoreductases, Oxidation-Reduction, Gene Deletion
Heme, Saccharomyces cerevisiae, Fungal Proteins, Cytochrome b562, Mice, Fumarates, Multienzyme Complexes, Animals, Lactic Acid, Mitochondrion, Electron Transport Complex II, Escherichia coli Proteins, Spectrum Analysis, Dithionite, Intracellular Membranes, Cytochrome b Group, Yeast, Malonates, Mitochondria, Succinate dehydrogenase, Succinate Dehydrogenase, Covalent flavin, Flavin-Adenine Dinucleotide, Fumarate-oxidizable, Oxidoreductases, Oxidation-Reduction, Gene Deletion
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