Characterization of KIBRA, a novel WW domain-containing protein
Copyright policy )Characterization of KIBRA, a novel WW domain-containing protein
In a yeast two hybrid screen with the human isoform of Dendrin (KIAA0749), a putative modulator of the postsynaptic cytoskeleton, we isolated a cDNA coding for a novel protein, KIBRA, possessing two amino-terminal WW domains, an internal C2-like domain and a carboxy-terminal glutamic acid-rich stretch. Northern blot analysis revealed that the expression of KIBRA mRNA was predominately found in kidney and brain. In vitro interaction studies revealed that the first KIBRA WW domain binds specifically to PPxY motifs. Transient transfection of monkey kidney cells with constructs encoding Myc-tagged KIBRA displayed a cytoplasmic localization and a perinuclear enrichment of the protein.
- Ludwig-Maximilians-Universität München Germany
- University of Münster Germany
- Anatomische Anstalt Germany
Recombinant Fusion Proteins, Molecular Sequence Data, Intracellular Signaling Peptides and Proteins, Proteins, Nerve Tissue Proteins, Phosphoproteins, Protein Structure, Tertiary, Two-Hybrid System Techniques, Chlorocebus aethiops, Mutagenesis, Site-Directed, Animals, Humans, Amino Acid Sequence, Sequence Alignment, Cells, Cultured
Recombinant Fusion Proteins, Molecular Sequence Data, Intracellular Signaling Peptides and Proteins, Proteins, Nerve Tissue Proteins, Phosphoproteins, Protein Structure, Tertiary, Two-Hybrid System Techniques, Chlorocebus aethiops, Mutagenesis, Site-Directed, Animals, Humans, Amino Acid Sequence, Sequence Alignment, Cells, Cultured
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