Functional Annotation of a Full-Length Arabidopsis cDNA Collection
Copyright policy )Functional Annotation of a Full-Length Arabidopsis cDNA Collection
Full-length complementary DNAs (cDNAs) are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. The 3′-end expressed sequence tags (ESTs) of 155,144 RAFL cDNAs were clustered into 14,668 nonredundant cDNA groups, about 60% of predicted genes. We also obtained 5′ ESTs from 14,034 nonredundant cDNA groups and constructed a promoter database. The sequence database of the RAFL cDNAs is useful for promoter analysis and correct annotation of predicted transcription units and gene products. Furthermore, the full-length cDNAs are useful resources for analyses of the expression profiles, functions, and structures of plant proteins.
- RIKEN Japan
- University of Tsukuba Japan
Expressed Sequence Tags, DNA, Complementary, DNA, Plant, Arabidopsis Proteins, Arabidopsis, Chromosome Mapping, Computational Biology, Gene Expression, Sequence Analysis, DNA, Genes, Plant, RNA, Messenger, Cloning, Molecular, Databases, Nucleic Acid, Promoter Regions, Genetic, Genome, Plant, Gene Library
Expressed Sequence Tags, DNA, Complementary, DNA, Plant, Arabidopsis Proteins, Arabidopsis, Chromosome Mapping, Computational Biology, Gene Expression, Sequence Analysis, DNA, Genes, Plant, RNA, Messenger, Cloning, Molecular, Databases, Nucleic Acid, Promoter Regions, Genetic, Genome, Plant, Gene Library
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