p150Glued, Dynein, and Microtubules Are Specifically Required for Activation of MKK3/6 and p38 MAPKs
Copyright policy )p150Glued, Dynein, and Microtubules Are Specifically Required for Activation of MKK3/6 and p38 MAPKs
To look for regulators of the mitogen-activated protein kinase (MAPK) kinase 6 (MKK6), a yeast two-hybrid screen was initiated using MKK6 as bait. p150(Glued) dynactin, a key component of the cytoplasmic dynein-dynactin motor complex, was found to specifically interact with MKK6 and its close homologue MKK3. Silencing of p150(Glued) expression by small interference RNA reduced the stimulus-induced phosphorylation of MKK3/6 and p38 MAPKs. The similar adverse effect was also seen when the cytoplasmic dynein motor was disrupted by other means. Like p150(Glued), MKK3/6 directly associate with microtubules. Disruption of microtubules prior to cell stimulation specifically inhibits the stimulus-induced phosphorylation of both MKK3/6 and p38 MAPKs. Our unexpected findings reveal a specific requirement for p150(Glued)/dynein/functional microtubules in activation of MKK3/6 and p38 MAPKs in vivo.
- Yale University United States
- Xiamen University China (People's Republic of)
- Hong Kong Polytechnic University China (People's Republic of)
- Southern Medical University China (People's Republic of)
- Hong Kong University of Science and Technology (香港科技大學) China (People's Republic of)
EXPRESSION, COMPLEX, 571, MAP Kinase Kinase 3, SIGNAL-TRANSDUCTION, PROTEIN, PATHWAYS, Dyneins, Dynactin Complex, MAP Kinase Kinase 6, Microtubules, p38 Mitogen-Activated Protein Kinases, TRANSPORT, Enzyme Activation, SUBUNIT, Humans, LIVING CELLS, Phosphorylation, Microtubule-Associated Proteins, DYNACTIN, HeLa Cells
EXPRESSION, COMPLEX, 571, MAP Kinase Kinase 3, SIGNAL-TRANSDUCTION, PROTEIN, PATHWAYS, Dyneins, Dynactin Complex, MAP Kinase Kinase 6, Microtubules, p38 Mitogen-Activated Protein Kinases, TRANSPORT, Enzyme Activation, SUBUNIT, Humans, LIVING CELLS, Phosphorylation, Microtubule-Associated Proteins, DYNACTIN, HeLa Cells
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).33 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!