The RETINOBLASTOMA-RELATED Gene Regulates Stem Cell Maintenance in Arabidopsis Roots
Copyright policy )The RETINOBLASTOMA-RELATED Gene Regulates Stem Cell Maintenance in Arabidopsis Roots
The maintenance of stem cells in defined locations is crucial for all multicellular organisms. Although intrinsic factors and signals for stem cell fate have been identified in several species, it has remained unclear how these connect to the ability to reenter the cell cycle that is one of the defining properties of stem cells. We show that local reduction of expression of the RETINOBLASTOMA-RELATED (RBR) gene in Arabidopsis roots increases the amount of stem cells without affecting cell cycle duration in mitotically active cells. Conversely, induced RBR overexpression dissipates stem cells prior to arresting other mitotic cells. Overexpression of D cyclins, KIP-related proteins, and E2F factors also affects root stem cell pool size, and genetic interactions suggest that these factors function in a canonical RBR pathway to regulate somatic stem cells. Expression analysis and genetic interactions position RBR-mediated regulation of the stem cell state downstream of the patterning gene SCARECROW.
- École Polytechnique Fédérale de Lausanne EPFL Switzerland
- Utrecht University Netherlands
- Delft University of Technology Netherlands
plant parenchyma cell, protein p57, Biomedical Research, gene overexpression, protein synthesis regulation, Arabidopsis, Down-Regulation, retinoblastoma protein, Plant Roots, Retinoblastoma Protein, Biologie/Milieukunde (BIOL), mitosis index, cyclin D, tumor suppressor gene, mitosis rate, Biochemistry, Genetics and Molecular Biology(all), Arabidopsis Proteins, gene interaction, plant root, Stem Cells, article, nucleotide sequence, Cell Differentiation, gene control, gene expression regulation, transcription factor E2F1, Life sciences, mitosis inhibition, cell size, gene function, priority journal, International (English), gene expression, cell cycle, somatic cell, Signal Transduction
plant parenchyma cell, protein p57, Biomedical Research, gene overexpression, protein synthesis regulation, Arabidopsis, Down-Regulation, retinoblastoma protein, Plant Roots, Retinoblastoma Protein, Biologie/Milieukunde (BIOL), mitosis index, cyclin D, tumor suppressor gene, mitosis rate, Biochemistry, Genetics and Molecular Biology(all), Arabidopsis Proteins, gene interaction, plant root, Stem Cells, article, nucleotide sequence, Cell Differentiation, gene control, gene expression regulation, transcription factor E2F1, Life sciences, mitosis inhibition, cell size, gene function, priority journal, International (English), gene expression, cell cycle, somatic cell, Signal Transduction
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).327 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!