Resonance assignments of the human AKAP13-PH domain and stabilizing DH helix
Copyright policy )Resonance assignments of the human AKAP13-PH domain and stabilizing DH helix
The human AKAP13 protein contains DH and PH domains, which are responsible for its cell transforming activity. Despite its biomedical importance, the contribution of the PH domain to AKAP13 activity remains unclear and no three dimensional structure is available to date. Here we report the backbone and side chain (1)H, (13)C and (15)N resonance assignments of a 20 kDa construct comprising the uniformly (13)C and( 15)N labeled AKAP13-PH domain and an associated helix from the DH domain which is required for its stable expression. Resonance assignment has been achieved using conventional triple resonance experiments; 95% of all back bone resonances and more than 90% of side chain resonances have been successfully assigned. The (1)H, (13)C and (15)N chemical shifts have been deposited in BMRB with accession number of 16195.
- Leibniz Association Germany
- University of Birmingham United Kingdom
- Leibniz Institute for Neurobiology Germany
- University of Oxford United Kingdom
Protein Stability, Molecular Sequence Data, A Kinase Anchor Proteins, Blood Proteins, Phosphoproteins, Protein Structure, Secondary, Protein Structure, Tertiary, DNA-Binding Proteins, Minor Histocompatibility Antigens, Proto-Oncogene Proteins, Humans, Amino Acid Sequence, Nuclear Magnetic Resonance, Biomolecular, Transcription Factors
Protein Stability, Molecular Sequence Data, A Kinase Anchor Proteins, Blood Proteins, Phosphoproteins, Protein Structure, Secondary, Protein Structure, Tertiary, DNA-Binding Proteins, Minor Histocompatibility Antigens, Proto-Oncogene Proteins, Humans, Amino Acid Sequence, Nuclear Magnetic Resonance, Biomolecular, Transcription Factors
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).2 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!