Protein Z/protein Z-dependent protease inhibitor system in loco in human gastric cancer
Copyright policy )Protein Z/protein Z-dependent protease inhibitor system in loco in human gastric cancer
In gastric cancer, hemostatic system components contribute to cancer progression, as activation of factor X (FX) was observed. The protein Z (PZ)/protein Z-dependent protease inhibitor (ZPI) complex inhibits factor Xa proteolytic activity. The purpose of this study was to determine the distribution of ZPI and PZ in relation to FX, and prothrombin fragment (F1 + 2), a standard marker for blood coagulation activation, in human gastric cancer tissue. ABC procedures and a double staining method employed polyclonal antibodies against PZ, FX, and F1 + 2 and a monoclonal antibody against ZPI. In situ hybridization (ISH) methods employed biotin-labeled 25-nucleotide single-stranded DNA probes directed to either PZ or ZPI mRNAs. FX and components of PZ/ZPI coagulation inhibitory system were observed in cancer cells. F1 + 2 was observed in gastric cancer cells as well. Double staining studies revealed FX/PZ, FX/ZPI, and PZ/ZPI co-localization on gastric cancer cells. ISH studies demonstrated the presence of PZ mRNA and ZPI mRNA in gastric cancer cells indicating induced synthesis of these proteins. The co-localization of PZ/ZPI and FX in gastric cancer cells indicates in loco that these proteins may play a role in anticoagulant events at the tumor tissue.
- University of New Mexico United States
- Wrocław Medical University Poland
- Medical University of Białystok Poland
messenger - metabolism, Adenocarcinoma - metabolism, Adenocarcinoma - genetics, Factor Xa - metabolism, Adenocarcinoma, Stomach neoplasms - pathology, Factor Xa - genetics, Prothrombin - metabolism, Stomach Neoplasms, Humans, RNA, Messenger, Protein Precursors, Prothrombin - genetics, Blood Coagulation, Blood proteins - genetics, In Situ Hybridization, Serpins, Gene expression regulation, Stomach neoplasms - genetics, Disease progression, messenger - genetics, Stomach neoplasms - metabolism, Hematology, Blood Proteins, Blood coagulation, Immunohistochemistry, Peptide Fragments, neoplastic, Peptide fragments - genetics, Gene Expression Regulation, Neoplastic, Serpins - metabolism, Protein precursors - metabolism, Blood proteins - metabolism, Peptide fragments - metabolism, Protein precursors - genetics, Factor Xa, Disease Progression, RNA, Original Article, Prothrombin, In situ hybridization, Adenocarcinoma - pathology, Serpins - genetics
messenger - metabolism, Adenocarcinoma - metabolism, Adenocarcinoma - genetics, Factor Xa - metabolism, Adenocarcinoma, Stomach neoplasms - pathology, Factor Xa - genetics, Prothrombin - metabolism, Stomach Neoplasms, Humans, RNA, Messenger, Protein Precursors, Prothrombin - genetics, Blood Coagulation, Blood proteins - genetics, In Situ Hybridization, Serpins, Gene expression regulation, Stomach neoplasms - genetics, Disease progression, messenger - genetics, Stomach neoplasms - metabolism, Hematology, Blood Proteins, Blood coagulation, Immunohistochemistry, Peptide Fragments, neoplastic, Peptide fragments - genetics, Gene Expression Regulation, Neoplastic, Serpins - metabolism, Protein precursors - metabolism, Blood proteins - metabolism, Peptide fragments - metabolism, Protein precursors - genetics, Factor Xa, Disease Progression, RNA, Original Article, Prothrombin, In situ hybridization, Adenocarcinoma - pathology, Serpins - genetics
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).14 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!