Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast
Copyright policy )Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast
We have expressed a bovine synthetic acyl-CoA-binding protein (ACBP) gene in yeast (Saccharomyces cerevisiae) under the control of the GAL1 promoter. The heterologously expressed bovine ACBP constituted up to 6.4% of total cellular protein and the processing was identical with that of native bovine ACBP, i.e. the initiating methionine was removed and the following serine residue was N-acetylated. The expression of this protein did not affect the growth rate of the cells. Determination of the yeast acyl-CoA pool size showed a close positive correlation between the ACBP content of the cells and the size of the acyl-CoA pool. Thus ACBP can act as an intracellular acyl-CoA pool former. Possible physiological functions of ACBP in cells are discussed.
- University of Southern Denmark Denmark
- Odense University Denmark
- Odense University Hospital Denmark
Base Sequence, Molecular Sequence Data, Oligonucleotides, Molecular, Saccharomyces cerevisiae, Fatty Acid-Binding Proteins, Recombinant Proteins, Neoplasm Proteins, Promoter Regions, Genetic, Escherichia coli, Animals, Cattle, Acyl Coenzyme A, Cloning, Molecular, Carrier Proteins, Promoter Regions, Genetic, Cloning, Plasmids
Base Sequence, Molecular Sequence Data, Oligonucleotides, Molecular, Saccharomyces cerevisiae, Fatty Acid-Binding Proteins, Recombinant Proteins, Neoplasm Proteins, Promoter Regions, Genetic, Escherichia coli, Animals, Cattle, Acyl Coenzyme A, Cloning, Molecular, Carrier Proteins, Promoter Regions, Genetic, Cloning, Plasmids
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