ABSTRACTAdult hippocampal neurogenesis (AHN) study is still a challenge. In addition to methodological difficulties is the controversy of results derived of human or animal system approaches. In view of the proven link between AHN and learning and memory impairment, we generated a straightforwardin vitromodel to recapitulate adult neurogenesis in the context of Alzheimer’s disease (AD).Neural progenitor cells (NPCs) monolayer culture was differentiated for a period of 29 days and Aβ peptide 1-42 was administered once a week. mRNA expression ofNEUROD1, NCAM1, TUBB3, RBFOX3, CALB1andGFAPgenes was determined by RT-qPCR.Phenotypic changes were observed during directed differentiation. Except forGFAPandCALB1, these changes correlated with altered expression profile of all genes since 9 days. OnlyTUBB3expression remained constant whileNEUROD1, NCAM1andRBFOX3expression increased over time. Moreover, Aβ treated NPCs showed transient decreases of mRNA expression forNCAM1, TUBB3andRBFOX3genes at 9 or 19 days.Ourin vitrohuman NPCs model is framed within the multistep process of AHN in the SGZ of the DG. Remarkably, its transcriptional assessment might reflect alterations detected in AD human patients, deepening our understanding of the disorder and possibly of its pathogenesis.SUMMARY STATEMENTTranscriptional profile of a number of genes recapitulating particular stages of Adult hippocampal neurogenesis in the context of Alzheimer’s disease