Genetic and physical characterization of a region of Arabidopsis chromosome 1 containing the CLAVATA1 gene
Genetic and physical characterization of a region of Arabidopsis chromosome 1 containing the CLAVATA1 gene
With the advance of Arabidopsis as a model system for understanding plant genetics, development and biochemistry, a detailed description of the genome is necessary. As such, focused projects are underway to map and sequence the Arabidopsis nuclear genome. We have characterized a region of chromosome 1, surrounding the CLAVATA1 (CLV1) locus. Three (RFLP) clones were mapped relative to clv1-1, and were used to construct an ca. 700 kb yeast artificial chromosome (YAC) contig. Three cosmids spanning the CLV1 locus were analyzed and ca. 24 kb of genomic DNA was sequenced, including a continuous stretch of 18 kb. In addition to generating clones in this region of chromosome 1, we have analyzed the size, spacing and organization of several contiguous genes.
- California Institute of Technology United States
- University of Michigan–Ann Arbor United States
- University of Michigan–Flint United States
- University of Michigan United States
YAC Contig, 570, DNA, Plant, Science, Ecology and Evolutionary Biology, Molecular Sequence Data, Restriction Mapping, YAC contig, Arabidopsis, Protein Serine-Threonine Kinases, Biochemistry, 630, Chromosomes, CLAVATA1, Health Sciences, Genetics, Phosphoprotein Phosphatases, Genome Sequencing, Cellular and Developmental Biology, General, Chromosomes, Artificial, Yeast, Arabidopsis Proteins, Plant Sciences, Life Sciences, Molecular, Natural Resources and Environment, Chromosome Mapping, Plant Pathology, genome sequencing, RFLP, Polymorphism, Restriction Fragment Length, Signal Transduction
YAC Contig, 570, DNA, Plant, Science, Ecology and Evolutionary Biology, Molecular Sequence Data, Restriction Mapping, YAC contig, Arabidopsis, Protein Serine-Threonine Kinases, Biochemistry, 630, Chromosomes, CLAVATA1, Health Sciences, Genetics, Phosphoprotein Phosphatases, Genome Sequencing, Cellular and Developmental Biology, General, Chromosomes, Artificial, Yeast, Arabidopsis Proteins, Plant Sciences, Life Sciences, Molecular, Natural Resources and Environment, Chromosome Mapping, Plant Pathology, genome sequencing, RFLP, Polymorphism, Restriction Fragment Length, Signal Transduction
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