The molecular cloning of a type II regulatory subunit of the cAMP-dependent protein kinase from rat skeletal muscle and mouse brain.
Copyright policy )The molecular cloning of a type II regulatory subunit of the cAMP-dependent protein kinase from rat skeletal muscle and mouse brain.
A cDNA clone for a type II regulatory (R) subunit of the cAMP-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37) was isolated from a rat skeletal muscle library using a specific 47-base oligonucleotide probe. The rat cDNA was 1.2 kilobases (kb) in length and contained an open reading frame of 1.113 kb representing 92% of the coding region of the molecule. Nick-translated rat cDNA was then used to isolate a mouse RII cDNA clone from a brain library that contained an open reading frame of 1.143 kb. Because both cDNAs lacked complete coding sequences, the remainder of the RII coding region was obtained from a 15-kb mouse genomic clone. The mouse RII coding region contains 1.2 kb corresponding to a 400-amino acid protein of 51.141 kDa. The mouse cDNA hybridizes to two mRNA species, a 2.4-kb form that was only observed in testis and a 6.0-kb form found in a wide range of tissues, including testis.
- University of Mary United States
- Howard Hughes Medical Institute United States
- Korea Advanced Institute of Science and Technology Korea (Republic of)
- Molecular and Behavioral Neuroscience Institute United States
- Cold Spring Harbor Laboratory United States
572, Base Sequence, Macromolecular Substances, Swine, Muscles, Brain, DNA Restriction Enzymes, Rats, Mice, Animals, Cattle, Amino Acid Sequence, Cloning, Molecular, Protein Kinases
572, Base Sequence, Macromolecular Substances, Swine, Muscles, Brain, DNA Restriction Enzymes, Rats, Mice, Animals, Cattle, Amino Acid Sequence, Cloning, Molecular, Protein Kinases
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