Amplification of the gene encoding the α-subunit of the mitochondrial ATP synthase complex in a human retinoblastoma cell line
Copyright policy )Amplification of the gene encoding the α-subunit of the mitochondrial ATP synthase complex in a human retinoblastoma cell line
A cDNA clone encoding the precursor of the alpha-subunit of the human mitochondrial ATP synthase (F1-ATPS) complex was isolated from a library prepared from the poly(A)+ RNA present in a retinoblastoma (RB) cell line. Northern blot analysis of RNAs derived from a variety of transformed cell lines as well as from normal human fetal tissues indicated that RNA expression was significantly higher in two of the four RB cell lines analysed, Y79 (10- to 30-fold) and RB522A (3- to 8-fold), than in other cell lines or tissues. The increased mRNA level was apparently the result of gene amplification in Y79, but not in RB522A.
- University of Alberta Canada
- Cross Cancer Institute Canada
- Cross Cancer Institute Canada
Base Sequence, Molecular Sequence Data, Gene Amplification, Retinoblastoma, Blotting, Northern, Mitochondria, Blotting, Southern, Proton-Translocating ATPases, Tumor Cells, Cultured, Humans, Amino Acid Sequence, Cloning, Molecular
Base Sequence, Molecular Sequence Data, Gene Amplification, Retinoblastoma, Blotting, Northern, Mitochondria, Blotting, Southern, Proton-Translocating ATPases, Tumor Cells, Cultured, Humans, Amino Acid Sequence, Cloning, Molecular
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