Characterization of a partial cDNA clone encoding porcine skeletal muscle titin: Comparison with rabbit and mouse skeletal muscle titin sequences
Copyright policy )Characterization of a partial cDNA clone encoding porcine skeletal muscle titin: Comparison with rabbit and mouse skeletal muscle titin sequences
1. A cDNA fragment encoding 571 amino acid residues of porcine skeletal muscle titin was isolated from total RNA using RNA-PCR. 2. The porcine titin clone hybridized to a large RNA species (> 23 kb) in rabbit cardiac muscle, rabbit skeletal muscle and porcine skeletal muscle. 3. The porcine skeletal muscle titin clone encoded two types of 100-residue motifs and its amino acid sequence was 96% and 93% identical to the corresponding sequence of rabbit and mouse skeletal muscle titin, respectively. These results suggest that the titin sequence is highly conserved among mammalian species.
- University of Wisconsin–Madison United States
- University of Wisconsin–Oshkosh United States
- University of Wisconsin System United States
Base Sequence, Swine, Molecular Sequence Data, Membrane Proteins, Muscle Proteins, Nucleic Acid Hybridization, DNA, Blotting, Northern, Polymerase Chain Reaction, Mice, Animals, Connectin, Amino Acid Sequence, Rabbits, Cloning, Molecular, Protein Kinases
Base Sequence, Swine, Molecular Sequence Data, Membrane Proteins, Muscle Proteins, Nucleic Acid Hybridization, DNA, Blotting, Northern, Polymerase Chain Reaction, Mice, Animals, Connectin, Amino Acid Sequence, Rabbits, Cloning, Molecular, Protein Kinases
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