PDCD4 is a CSL associated protein with a transcription repressive function in cancer associated fibroblast activation
Copyright policy )PDCD4 is a CSL associated protein with a transcription repressive function in cancer associated fibroblast activation
The Notch/CSL pathway plays an important role in skin homeostasis and carcinogenesis. CSL, the key effector of canonical Notch signaling endowed with an intrinsic transcription repressive function, suppresses stromal fibroblast senescence and Cancer Associated Fibroblast (CAF) activation through direct down-modulation of key effector genes. Interacting proteins that participate with CSL in this context are as yet to be identified. We report here that Programmed Cell Death 4 (PDCD4), a nuclear/cytoplasmic shuttling protein with multiple functions, associates with CSL and plays a similar role in suppressing dermal fibroblast senescence and CAF activation. Like CSL, PDCD4 is down-regulated in stromal fibroblasts of premalignant skin actinic keratosis (AKs) lesions and squamous cell carcinoma (SCC). While devoid of intrinsic DNA binding capability, PDCD4 is present at CSL binding sites of CAF marker genes as well as canonical Notch/CSL targets and suppresses expression of these genes in a fibroblast-specific manner. Thus, we propose that PDCD4 is part of the CSL repressive complex involved in negative control of stromal fibroblasts conversion into CAFs.
- Massachusetts General Hospital United States
- Harvard Medical School United States
- University of Lausanne Switzerland
- Harvard University United States
- Department of Biochemistry Switzerland
Skin Neoplasms, Transcription, Genetic, 610, Down-Regulation, Mice, SCID, squamous cancer, Mice, Animals; Apoptosis Regulatory Proteins/genetics; Apoptosis Regulatory Proteins/metabolism; Cancer-Associated Fibroblasts/immunology; Cancer-Associated Fibroblasts/metabolism; Carcinoma, Squamous Cell/genetics; Carcinoma, Squamous Cell/metabolism; Cell Line, Tumor; Cellular Senescence; Down-Regulation; HEK293 Cells; HeLa Cells; Humans; Immunoglobulin J Recombination Signal Sequence-Binding Protein/metabolism; Keratosis, Actinic/genetics; Keratosis, Actinic/metabolism; Mice; Mice, SCID; Protein Binding; RNA, Small Interfering/genetics; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Signal Transduction; Skin Neoplasms/genetics; Skin Neoplasms/metabolism; Transcription, Genetic; Xenograft Model Antitumor Assays; CAFs; Notch/CSL signaling; PDCD4; squamous cancer; transcription repression, Cancer-Associated Fibroblasts, Cell Line, Tumor, Animals, Humans, RNA, Small Interfering, Cellular Senescence, Notch/CSL signaling, PDCD4, CAFs, RNA-Binding Proteins, transcription repression, Keratosis, Actinic, HEK293 Cells, Immunoglobulin J Recombination Signal Sequence-Binding Protein, Carcinoma, Squamous Cell, Apoptosis Regulatory Proteins, Priority Research Paper, HeLa Cells, Protein Binding, Signal Transduction
Skin Neoplasms, Transcription, Genetic, 610, Down-Regulation, Mice, SCID, squamous cancer, Mice, Animals; Apoptosis Regulatory Proteins/genetics; Apoptosis Regulatory Proteins/metabolism; Cancer-Associated Fibroblasts/immunology; Cancer-Associated Fibroblasts/metabolism; Carcinoma, Squamous Cell/genetics; Carcinoma, Squamous Cell/metabolism; Cell Line, Tumor; Cellular Senescence; Down-Regulation; HEK293 Cells; HeLa Cells; Humans; Immunoglobulin J Recombination Signal Sequence-Binding Protein/metabolism; Keratosis, Actinic/genetics; Keratosis, Actinic/metabolism; Mice; Mice, SCID; Protein Binding; RNA, Small Interfering/genetics; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Signal Transduction; Skin Neoplasms/genetics; Skin Neoplasms/metabolism; Transcription, Genetic; Xenograft Model Antitumor Assays; CAFs; Notch/CSL signaling; PDCD4; squamous cancer; transcription repression, Cancer-Associated Fibroblasts, Cell Line, Tumor, Animals, Humans, RNA, Small Interfering, Cellular Senescence, Notch/CSL signaling, PDCD4, CAFs, RNA-Binding Proteins, transcription repression, Keratosis, Actinic, HEK293 Cells, Immunoglobulin J Recombination Signal Sequence-Binding Protein, Carcinoma, Squamous Cell, Apoptosis Regulatory Proteins, Priority Research Paper, HeLa Cells, Protein Binding, Signal Transduction
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