Mammalian and yeast U3 snoRNPs are matured in specific and related nuclear compartments
Copyright policy )Mammalian and yeast U3 snoRNPs are matured in specific and related nuclear compartments
Nucleolar localization of vertebrate box C/D snoRNA involves transit through Cajal bodies, but the significance of this event is unknown. To define better the function of this compartment, we analyzed here the maturation pathway of mammalian U3. We show that 3'-extended U3 precursors possess a mono-methylated cap, and are not associated with fibrillarin and hNop58. Importantly, these precursors are detected at both their transcription sites and in Cajal bodies. In addition, mature U3, the core box C/D proteins and the human homolog of the methyltransferase responsible for U3 cap tri-methylation, hTgs1, are all present in Cajal bodies. In yeast, U3 follows a similar maturation pathway, and equivalent 3'-extended precursors are enriched in the nucleolus and in the nucleolar body, a nucleolar domain that concentrates Tgs1p under certain growth conditions. Thus, spatial organization of U3 maturation appears to be conserved across evolution, and involves specialized and related nuclear compartments, the nucleolus/nucleolar body in yeast and Cajal bodies in higher eukaryotes. These are likely places for snoRNP assembly, 3' end maturation and cap modification.
- Sorbonne University France
- Université Libre de Bruxelles Belgium
- University of Paris France
- Institut des Sciences Biologiques France
- French National Centre for Scientific Research France
Cell Nucleus -- metabolism, Chromosomal Proteins, Non-Histone, Xenopus, Fluorescence Nuclear Proteins/metabolism Nucleic Acid Conformation Plasmids/metabolism Precipitin Tests Protein Binding Protein Structure, DNA -- metabolism, Ribonucleoproteins, Small Nucleolar, In Situ Hybridization, Animals Cell Line Cell Nucleolus/metabolism Cell Nucleus/*metabolism Chromosomal Proteins, Microscopy, Non-Histone/metabolism Coiled Bodies/metabolism DNA/metabolism DNA Methylation Hela Cells Humans In Situ Hybridization Microscopy, Nuclear Proteins, Non-Histone -- metabolism, Small Nucleolar -- metabolism -- physiology, Chromosomal Proteins, Ribonucleoproteins, Plasmids -- metabolism, Cell Nucleolus, Plasmids, Protein Binding, Protein Structure, Saccharomyces cerevisiae Proteins, Cell Nucleolus -- metabolism, Coiled Bodies, Saccharomyces cerevisiae, Fluorescence, Cell Line, Tertiary Ribonucleoproteins, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Animals, Humans, Saccharomyces cerevisiae -- metabolism, Cell Nucleus, Small Nucleolar/*metabolism/*physiology Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins Xenopus, synthèse des protéines, Biologie moléculaire, DNA, DNA Methylation, Precipitin Tests, Coiled Bodies -- metabolism, Protein Structure, Tertiary, Microscopy, Fluorescence, Nucleic Acid Conformation, Biologie cellulaire, Nuclear Proteins -- metabolism, Acides nucléiques, Tertiary, HeLa Cells
Cell Nucleus -- metabolism, Chromosomal Proteins, Non-Histone, Xenopus, Fluorescence Nuclear Proteins/metabolism Nucleic Acid Conformation Plasmids/metabolism Precipitin Tests Protein Binding Protein Structure, DNA -- metabolism, Ribonucleoproteins, Small Nucleolar, In Situ Hybridization, Animals Cell Line Cell Nucleolus/metabolism Cell Nucleus/*metabolism Chromosomal Proteins, Microscopy, Non-Histone/metabolism Coiled Bodies/metabolism DNA/metabolism DNA Methylation Hela Cells Humans In Situ Hybridization Microscopy, Nuclear Proteins, Non-Histone -- metabolism, Small Nucleolar -- metabolism -- physiology, Chromosomal Proteins, Ribonucleoproteins, Plasmids -- metabolism, Cell Nucleolus, Plasmids, Protein Binding, Protein Structure, Saccharomyces cerevisiae Proteins, Cell Nucleolus -- metabolism, Coiled Bodies, Saccharomyces cerevisiae, Fluorescence, Cell Line, Tertiary Ribonucleoproteins, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Animals, Humans, Saccharomyces cerevisiae -- metabolism, Cell Nucleus, Small Nucleolar/*metabolism/*physiology Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins Xenopus, synthèse des protéines, Biologie moléculaire, DNA, DNA Methylation, Precipitin Tests, Coiled Bodies -- metabolism, Protein Structure, Tertiary, Microscopy, Fluorescence, Nucleic Acid Conformation, Biologie cellulaire, Nuclear Proteins -- metabolism, Acides nucléiques, Tertiary, HeLa Cells
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