Fibulin-2 is present in murine vascular lesions and is important for smooth muscle cell migration
Copyright policy )Fibulin-2 is present in murine vascular lesions and is important for smooth muscle cell migration
The vascular extracellular matrix (ECM) can affect smooth muscle cell (SMC) adhesion, migration and proliferation-events that are important during the atherosclerotic process. Fibulin-2 is a member of the ECM protein family of fibulins and has been found to cross-link versican/hyaluronan complexes, an ECM network that has been suggested to be important during tissue repair. In this study we have analysed the presence of fibulin-2 in two different models of murine vascular lesions. We have also examined how the fibulin-2/versican network influences SMC migration.Presence of fibulin-2 was analysed by immunohistochemistry in atherosclerotic aortas and in mechanically injured carotid arteries from mice. Fibulin-2 protein levels were also studied by Western blotting during rat aortic SMC phenotypic modulation in vitro. The importance of a fibulin-2/versican interaction for SMC migration was studied in the presence of two inhibiting peptides (FN III 3-5 and aggrecan C-type lectin-like domain).Fibulin-2 is expressed in SMC rich regions of atherosclerotic lesions where it colocalises with versican and hyaluronan. It is also present in injury-induced vascular lesions and is upregulated during SMC phenotypic modulation in cell culture. Moreover, treatments with peptides that block the interaction between versican and fibulin-2 inhibit SMC migration in vitro.Fibulin-2 can be produced by SMC as a response to injury and may participate in the ECM organisation that regulates SMC migration during vessel wall repair.
- University of Copenhagen Denmark
- Lund University Sweden
- University of Copenhagen Denmark
Mice, Knockout, Extracellular Matrix Proteins, Reverse Transcriptase Polymerase Chain Reaction, Blotting, Western, Calcium-Binding Proteins, Atherosclerosis, Muscle, Smooth, Vascular, Rats, Mice, Inbred C57BL, Mice, Apolipoproteins E, Chondroitin Sulfate Proteoglycans, Receptors, LDL, Cell Movement, Animals, Lectins, C-Type, Aggrecans, Hyaluronic Acid, Peptides, Cells, Cultured
Mice, Knockout, Extracellular Matrix Proteins, Reverse Transcriptase Polymerase Chain Reaction, Blotting, Western, Calcium-Binding Proteins, Atherosclerosis, Muscle, Smooth, Vascular, Rats, Mice, Inbred C57BL, Mice, Apolipoproteins E, Chondroitin Sulfate Proteoglycans, Receptors, LDL, Cell Movement, Animals, Lectins, C-Type, Aggrecans, Hyaluronic Acid, Peptides, Cells, Cultured
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