Inhibiting Crm1 causes the formation of excess acentriolar spindle poles containing NuMA and B23, but does not affect centrosome numbers
Copyright policy )Inhibiting Crm1 causes the formation of excess acentriolar spindle poles containing NuMA and B23, but does not affect centrosome numbers
Background. B23/nucleophosmin is present on spindle poles at metaphase. Migration of B23 to the poles is under the control of exportin Crm1. B23 at the centrosome plays a role in the control centrosome duplication.Results. h‐Tert‐RPE1 cells blocked in prometaphase with low doses of Nocodazol showed a progression to mitosis if Crm1 exportin was inhibited. Under these conditions, the formation of accessory poles containing γ‐tubulin, NuMA (nuclear‐mitotic‐apparatus) and B23 was induced at metaphase. No effect on centrosome number was observed. In quiescent h‐Tert‐RPE1 cells, when Crm1 was active, B23 was not detected at the centrosome as well as B23‐mutants reported to block centrosome duplication. In addition, the modification of B23 nucleo‐cytoplasmic shuttling showed no effect on centrosome duplication.Conclusion. Inhibition of Crm1 in early metaphase favours the formation of supplementary acentriolar spindle poles. B23 and NuMA are present at these poles that ultimately focus around the centrosome. Inhibition of Crm1 at metaphase has no effect on the control of centrosome numbers.
- Institute Curie France
Centrosome, Down-Regulation, Nuclear Proteins, Receptors, Cytoplasmic and Nuclear, Antigens, Nuclear, Cell Cycle Proteins, Exportin 1 Protein, Spindle Apparatus, Karyopherins, Cell Line, Mice, Protein Transport, Nuclear Matrix-Associated Proteins, Animals, Humans, Nucleophosmin, Metaphase
Centrosome, Down-Regulation, Nuclear Proteins, Receptors, Cytoplasmic and Nuclear, Antigens, Nuclear, Cell Cycle Proteins, Exportin 1 Protein, Spindle Apparatus, Karyopherins, Cell Line, Mice, Protein Transport, Nuclear Matrix-Associated Proteins, Animals, Humans, Nucleophosmin, Metaphase
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).10 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!