Identification of New Mediator Subunits in the RNA Polymerase II Holoenzyme from Saccharomyces cerevisiae
Copyright policy )Identification of New Mediator Subunits in the RNA Polymerase II Holoenzyme from Saccharomyces cerevisiae
Mediator was isolated from yeast on the basis of its requirement for transcriptional activation in a fully defined system. We have now identified three new members of mediator in the low molecular mass range by peptide sequence determination. These are the products of the NUT2, CSE2, and MED11 genes. The product of the NUT1 gene is evidently a component of mediator as well. NUT1 and NUT2 were earlier identified as negative regulators of the HO promoter, whereas mutations in CSE2 affect chromosome segregation. MED11 is a previously uncharacterized gene. The existence of these proteins in the mediator complex was verified by copurification and co-immunoprecipitation with RNA polymerase II holoenzyme.
- University of Gothenburg Sweden
- Stanford University United States
Mediator Complex, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Molecular Sequence Data, Nuclear Proteins, Saccharomyces cerevisiae, Precipitin Tests, Fungal Proteins, Amino Acid Sequence, RNA Polymerase II, Holoenzymes, Sequence Analysis, Protein Binding, Transcription Factors
Mediator Complex, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Molecular Sequence Data, Nuclear Proteins, Saccharomyces cerevisiae, Precipitin Tests, Fungal Proteins, Amino Acid Sequence, RNA Polymerase II, Holoenzymes, Sequence Analysis, Protein Binding, Transcription Factors
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