Copyright information:Taken from "DNA bending by bHLH charge variants"Nucleic Acids Research 2006;34(17):4846-4856.Published online 14 Sep 2006PMCID:PMC1635255.© 2006 The Author(s) () Design of phasing probes 1–5. Phasing probes derived from previous constructs (Ref) were modified to contain the E-box sequence required for Pho4p binding. All probes also contained an array of three phased A-tracts. The distance in bp between the Pho4p binding site and A-tracts array varies as shown. () Typical autoradiogram obtained after 8% native polyacrylamide gel electrophoresis of phasing probes 1–5 incubated with the bHLH charge variants (neutral: SAA, anionic: EEE, cationic: KKK). () As in (B), except phasing probes were incubated with bHLH charge variants. () Phasing analysis of electrophoretic data for probes 1–5 both free and bound to charge variants (SAA, EEE and KKK) of both bHLH peptide constructs. The relative mobilities of each of the five phasing probes in a given condition (free, bound to SAA, EEE, KKK) were plotted as a function of the spacing between the center of the curvature in the proximal A tract and the middle of the E-box binding site (). Data were fit to as described in Materials and Methods.