The products ofYCF1 andYLL015w (BPT1) cooperate for the ATP-dependent vacuolar transport of unconjugated bilirubin inSaccharomyces cerevisiae
Copyright policy )The products ofYCF1 andYLL015w (BPT1) cooperate for the ATP-dependent vacuolar transport of unconjugated bilirubin inSaccharomyces cerevisiae
Since bilirubin-like pigments are present in the environment as degradation products of heme-containing proteins, yeast could have developed a detoxifying system to transport these compounds into their vacuoles. Vacuoles from Saccharomyces cerevisiae showed an ATP-dependent, saturative transport of unconjugated bilirubin (UCB) that was reduced by 60% and 40% in YCF1 and YLL015w-deleted cells, respectively; the double deletant showed no UCB uptake. Conversely, the transport of bile acids (taurocholate) was comparable in wild and deleted stains. These data identify YCF1 and YLL015w, named BPT1 (Bile Pigment Transporter), as the genes responsible for ATP-dependent UCB transport in yeast. Since YCF1 and YLL015w are rather homologous with multidrug resistant proteins (MRPs), they also suggest the involvement of this class of transporters in the ATP-dependent transport of unconjugated bilirubin.
- University of Trieste Italy
- Université Catholique de Louvain Belgium
- VA Puget Sound Health Care System United States
- VA Northwest Network United States
- University of Washington United States
Saccharomyces cerevisiae Proteins, Time Factors, YCF1, Molecular Sequence Data, Calcium-Transporting ATPases, Saccharomyces cerevisiae, yeast, bilirubin; yeast; YCF1; ABC transporters; MRPs, Fungal Proteins, Adenosine Triphosphate, Amino Acid Sequence, Sequence Homology, Amino Acid, MRPs, Bilirubin, Biological Transport, Kinetics, ABC transporters, Mutation, Vacuoles, ATP-Binding Cassette Transporters, bilirubin, Sodium-Potassium-Exchanging ATPase, Vanadates, Gene Deletion
Saccharomyces cerevisiae Proteins, Time Factors, YCF1, Molecular Sequence Data, Calcium-Transporting ATPases, Saccharomyces cerevisiae, yeast, bilirubin; yeast; YCF1; ABC transporters; MRPs, Fungal Proteins, Adenosine Triphosphate, Amino Acid Sequence, Sequence Homology, Amino Acid, MRPs, Bilirubin, Biological Transport, Kinetics, ABC transporters, Mutation, Vacuoles, ATP-Binding Cassette Transporters, bilirubin, Sodium-Potassium-Exchanging ATPase, Vanadates, Gene Deletion
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